Document 0468
 DOCN  M9480468
 TI    Identification and analysis of the pseudoknot-containing gag-pro
       ribosomal frameshift signal of simian retrovirus-1.
 DT    9410
 AU    ten Dam E; Brierley I; Inglis S; Pleij C; Leiden Institute of Chemistry,
       Department of Biochemistry,; Gorlaeus Laboratories, Leiden University,
       The Netherlands.
 SO    Nucleic Acids Res. 1994 Jun 25;22(12):2304-10. Unique Identifier :
       AIDSLINE MED/94310058
 AB    The pro and pol genes of simian retrovirus-1 (SRV-1) are expressed as
       parts of a fusion protein generated by -1 ribosomal frameshifting. To
       investigate the requirements for frameshifting at the gag-pro overlap,
       we have inserted a stretch of 58 nucleotides containing the proposed
       frameshift signal into a plasmid that allows monitoring of translation
       in all three reading frames. In vitro translation of mRNAs derived from
       this plasmid indicated that the 58 nucleotides from the SRV-1 gag-pro
       overlap were sufficient to induce an efficient -1 shift in a
       heterologous context. Mutational analysis demonstrated that the slip
       site is formed at the heptanucleotide G GGA AAC. The frameshift
       efficiency of the wild type sequence in rabbit reticulocyte lysate was
       23%. A second component of the frameshift signal is formed by a
       pseudoknot seven bases downstream of the slip site. The presence of this
       pseudoknot was confirmed by mutational analysis, employing complementary
       and compensatory base changes, and by probing the structure of short RNA
       transcripts containing the frameshift signal. Adding increasing amounts
       of an SRV-1 pseudoknot containing RNA transcript to a translation
       reaction programmed with an SRV-1 frameshift reporter mRNA had no effect
       on the frameshift efficiency, arguing against the role of a specific
       pseudoknot-recognising factor in the frameshifting process.
 DE    Base Sequence  Cloning, Molecular  Frameshift Mutation  *Gene Expression
       Regulation, Viral  *Genes, gag  *Genes, pol  Molecular Sequence Data
       Nucleic Acid Conformation  Regulatory Sequences, Nucleic Acid
       Retroviruses Type D, Simian/*GENETICS  RNA, Viral/CHEMISTRY/*GENETICS
       Support, Non-U.S. Gov't  Translation, Genetic  Viral Proteins/GENETICS
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).